Titlebook: Somatic Genome Manipulation; Advances, Methods, a Xiu-Qing Li,Danielle J. Donnelly,Thomas G. Jensen Book 2015 Springer Science+Business Med

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Targeted Porcine Genome Engineering with TALENscol, including construction of sequence-specific TALENs, delivery of TALENs into primary porcine fibroblasts, and detection of TALEN-mediated cleavage, is described. This chapter is useful for scientists who are inexperienced with TALEN engineering of porcine cells as well as of other large animals.

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Somatic Gene Therapy Using Viral Vectors: Theoretical and Clinical Implications in Relation to Treat art vector technology applied in clinical trials, present the development of ocular gene therapy vectors in terms of efficacy, safety, and persistency, and finally, give an overview of some of the more notable advances in somatic gene therapy using viral vectors for treatment of inherited eye disea

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Nonviral Gene Therapy—The Challenge of Mobilizing DNAhe nuclei of desired cells, additional action is required. Such actions may serve to increase cell permeability, for example by exposing target cells to high pressure or an externally applied electrical field. Other strategies aim at mimicking viral gene transfer by engineering synthetic particles o

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Dendritic-Cells

Somatic Cell Nuclear Transfer and the Creation of Transgenic Large Animal Modelsnimals, for which embryonic stem cells that can be cultured and reliably modified in the laboratory are not fully established. In addition, new gene-editing technologies, such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs), have further assisted the crea

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Spina-Bifida

Protoplast Technology in Genome Manipulation of Potato Through Somatic Cell Fusionr ones, whereas asymmetric fusion allows partial genome transfer from a donor cell to a recipient. In asymmetric fusion, the donor cells are usually subjected to irradiation or ultraviolet treatment before fusion. In comparison to other techniques of chromosomal and genetic engineering, somatic geno

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Mitochondrial Genetic Manipulationlear expression and mitochondrial trafficking of proteins or RNAs through different targeting and shuttling systems have been widely developed to circumvent the need for regular transformation. A number of these methodologies were reported to be successful, for instance to rescue pathogenic mutation

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