Titlebook: Somatic Embryogenesis; Methods and Protocol Marco A. Ramírez-Mosqueda Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Somatic Embryogenesis in Sugarcane (, spp.)ality emblings. In this chapter, we describe a detailed protocol for somatic embryogenesis in sugarcane (. sp.) to be used in research projects for small-scale production. This protocol comprises all steps from explant preparation to the establishment of sugarcane emblings.

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几何学家

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Book 2022ic embryogenesis. Chapters guide readers through somatic embryogenesis protocols, somatic embryos in plants, and new methodologies that are used out to solve in vitro recalcitrance of many species to this morphogenetic route. Written in the format of the highly successful .Methods in Molecular Biolo

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: Somatic Embryogenesisevelop secondary embryos. This step of indirect SE allows us to produce large quantities of embryos and to do mass propagation using liquid culture medium. Despite a very strong clone dependency and high batch-to-batch variability, about 80% of . cultivars respond to somatic embryogenesis and can be propagated by this method.

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Somatic Embryogenesis in Citrus (, spp.), var. Valenciaue and the use of an alternative method of callus sieving for culture synchronization and embryo production. This is a simple method which can also be evaluated for other species aiming at better culture efficiency and somatic embryo production.

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不近人情

Citrus Protoplast Isolation and Plant Regeneration Through Somatic Embryogenesis is an important methodology for breeding applications. The methods presented here can also be used as a reference for protoplast work in other species, followed by protocol optimization for different species/genotypes.

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