Titlebook: Somatic Cell Genetics; C. Thomas Caskey,D. Christopher Robbins Book 1982 Springer Science+Business Media New York 1982 cell.cells.chromoso

irradicable

Dihydrofolate Reductase Gene Amplification, Altered Dihydrofolate Reductase, and Methotrexate Resisnding increase in the number of DHFR genes, i.e., gene amplification. irrespective of whether the karyotype is grossly aneuploid or is relatively stable, and irrespective of whether the MTX-resistance is phenotypically stable or unstable.

Obedient

Spinal-Fusion

斑驳

Isolation and Analysis of Chinese Hamster Cells Carrying Forward and Reverse Mutations in the Hypox considerable attention (see Caskey and Kruh for a review.. The product of this gene in various mammalian systems is a protein of about 25,000 molecular weight which associates into oligomers that catalyze the formation of IMP or GMP from 5-phosphoribosyl pyrophosphate (PP-Ribose-P) plus hypoxanthin

未成熟

Towards Mapping Genes to Chromosomes Using Two Dimensional Gel Electrophoresis of Proteins and Somaomosomes or to regions of chromosomes.. This approach, however, is limited by the small number of genetic markers available for the selection of hybrids. and more seriously by the lack of sensitive assays to determine how much information is coexpressed in the hybrids at the molecular level..

mucous-membrane

ventilate

Lamina

Dihydrofolate Reductase Gene Amplification, Altered Dihydrofolate Reductase, and Methotrexate Resisvestigated the development of resistance to the 4-amino analog of folate, methotrexate (MTX) and have defined three general mechanisms for such resistance, including reduction in MTX transport,. alteration in affinity of MTX for the target enzyme of MTX inhibition, dihydrofolate reductase (DHFR). an

出处

Transformation and Expression of TK Sequences,n in HAT medium (1,2,3,). These experiments suggested to us that isolation of the virus sequences encoding tk was feasible. Accordingly, virus DNA cleaved with a variety of restriction endonucleases was assayed for its ability to convert tk. cells to a HAT resistant phenotype using the calcium phosp

轻信

Genetic Variants of Cultured Animal Cells, of mutants of broad phenotypic classes. It was with this understanding that our laboratory undertook about ten years ago to develop methods for selection of mutants in somatic cells. Over this decade the field has developed extremely rapidly both in terms of methodological expertise and in the wide