Titlebook: Somaclonal Variation: Basic and Practical Aspects; Carolina Sánchez-Romero Book 2024 The Editor(s) (if applicable) and The Author(s), unde

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nalysis.The occurrence of somaclonal variation in relevant sThis book is fundamental for plant biotechnologists at this moment, due to the extensive use of in vitro culture. Although some books about somaclonal variation were published some years ago, the circumstances in this field have undergone a

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Epigenetic Basis of Somaclonal Variation,y. These changes affect chromatin structure and include changes in DNA methylation, histone modifications and the expression of small RNAs involved in DNA methylation (RNA-directed DNA methylation; RdDM). DNA methylation is one of the best studied epigenetic marks in regenerated plants, and changes

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Factors Affecting Tissue Culture-Induced Variations,generated plants and their progenies. A wide range of alterations can emerge as a consequence of somaclonal variation, which can be detected at different levels, such as phenotypic, cytogenetic, biochemical or molecular. Nature and frequency of changes are uncontrolled due to the unpredictable natur

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Molecular Markers for the Detection and Analysis of Somaclonal Variation,issue culture. Different molecular markers based on PCR provide useful tools to check the genetic and epigenetic variations associated to in vitro culture. Arbitrary amplifications, such as those obtained through RAPD, ISSR and AFLP markers, allow the assessment of the appearance of somaclonal varia

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Somaclonal Variation in Conifers,es, junipers, pines, and redwoods, to name a few important constituents. By studying diverse facets of conifer tissue culture, our understanding of the key factors responsible for enhancing the quality and quantity of production might potentially be elucidated in this group. Furthermore, this can pr