Titlebook: Single Stranded DNA Binding Proteins; Marcos T. Oliveira Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 ssDN

导师

Expression, Purification, and Solution-State NMR Analysis of the Two Human Single-Stranded DNA-Bindhe molecular and structural details of both hSSBs and their interactions with DNA and RNA. Here we provide a detailed overview of how to express and purify recombinant versions of these important human proteins for the purpose of detailed structural analysis by high-resolution solution-state NMR.

DEFT

,Analysis of Mitochondrial SSB-DNA Complexes and Their Effects on DNA Polymerase γ Activity by Electmulatory properties of mtSSB result from its ability to organize the single-stranded DNA template in a specific manner. Here we present methods employing electron microscopy and enzymatic assays to characterize and classify the mtSSB-DNA complexes and their effects on the activity of Pol γ.

hankering

Measurements of Real-Time Replication Kinetics of DNA Polymerases on ssDNA Templates Coated with Sit the single-molecule level the effect of single-stranded DNA-binding proteins (SSB) on the real-time replication kinetics of the human mitochondrial DNA polymerase during the synthesis of the lagging strand.

水汽

Antarctic

慢慢流出

Mets552

Single-Molecule Fluorescence Methods to Study Protein Exchange Kinetics in Supramolecular ComplexesProteins can dynamically exchange in and out of the complex depending on their concentration in solution. These exchange dynamics are a key determinant of the molecular pathways available to multi-protein complexes. It is therefore important that we develop robust and reproducible assays to study pr

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Comparing SSB-PriA Functional and Physical Interactions in Gram-Positive and -Negative Bacteria,e the SSB interactome. The mechanism through which PriA helicase, an initiator protein in the DNA replication restart process, is stimulated by SSB in . (EcSSB) has been established. However, some Gram-positive bacterial SSBs such as . SsbA (a counterpart of EcSSB), . SsbA, SsbB, and SsbC do not act

伪书

中子

Magnetic Tweezers-Based Single-Molecule Assays to Study Interaction of , SSB with DNA and RecQ Helis involved in various aspects of nucleic acid metabolism. These studies have led to the discovery of key aspects of protein-protein and protein-nucleic acid interaction, uncovering dynamic heterogeneities that are lost to ensemble averaging in bulk experiments. The versatility of magnetic tweezers l