书目名称 | Single Molecule Enzymology | 副标题 | Methods and Protocol | 编辑 | Gregory I. Mashanov,Christopher Batters | 视频video | | 概述 | Serves as an ideal guide for the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail.Features cutting-edge, lab ready protocols.Includes key tips and e | 丛书名称 | Methods in Molecular Biology | 图书封面 |  | 描述 | .The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In .Single Molecule Enzymology: Methods and Protocols., experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful .Methods in Molecular Biology™. series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. .Authoritative and up-to-date, .Single Molecule Enzymology: Meth | 出版日期 | Book 2011 | 关键词 | Cell signaling; Data analysis; Enzymes; Sample preparation; Single molecule measurements | 版次 | 1 | doi | https://doi.org/10.1007/978-1-61779-261-8 | isbn_softcover | 978-1-4939-6190-0 | isbn_ebook | 978-1-61779-261-8Series ISSN 1064-3745 Series E-ISSN 1940-6029 | issn_series | 1064-3745 | copyright | Springer Science+Business Media, LLC 2011 |
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