Titlebook: Single Cell ‘Omics of Neuronal Cells; Jonathan V. Sweedler,James Eberwine,Scott E. Frase Book 2022 The Editor(s) (if applicable) and The A

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High-Throughput Image-Guided Microprobe Mass Spectrometric Analysis of Single Cells,ly information-rich techniques available. There are two major approaches for characterizing single cells using MS: the assay of small-volume solutions using electrospray ionization and, the focus of this chapter, measurement of cells dispersed across a surface using matrix-assisted laser desorption/

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Quantifying Enzyme Activity and Gene Expression Within Single Cells Using a Multiplexed Capillary Eg creative combinations of chemical sensors with separation methods and detection instrumentation. However, despite many impressive developments over the past decade, the scope of chemical cytometry methods has remained limited, in part due to a reliance on the use of chemical sensors for analyte de

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Quantitative Chemical Imaging at the Cellular Level: SIMS, Fluorescence, and Correlative Techniquesing at the cellular level enables the investigation of the structural and functional molecular relation underlying cellular processes. We describe here the detailed methodology of the state-of-the-art secondary ion mass spectrometry (SIMS, NanoSIMS) and fluorescence microscopy (confocal, STED), alon

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Genetically Encoded Biosensors Reveal Spatiotemporal Dynamics and Cellular Heterogeneity of Neuronaular changes in second messenger levels and enzymatic activities, the biochemical architecture of neuronal cells is in a constant state of flux. Biochemical dynamics are at the root of such complex processes such as neuronal development, learning and memory, and behavior. Noninvasively measuring and

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