Titlebook: Signal Transduction Protocols; Robert C. Dickson,Michael D. Mendenhall Book 20042nd edition Humana Press 2004

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Determining Protein Half-Lives,ing the turnover rate of a protein is often the first step in assessing whether or not the function of a protein is regulated by proteolysis under specific physiological conditions. Over the years, procedures to determine the half-life of proteins in cultured eukaryotic cells have been well-establis

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Characterization of Protein-DNA Association In Vivo by Chromatin Immunoprecipitation,c regions in the context of intact cells. In this method, cells are first treated with formaldehyde to crosslink protein-protein and protein-DNA complexes .. Next, the crosslinked chromatin is sheared by sonication to generate small chromatin fragments, and the fragments associated with the protein

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Nucleocytoplasmic Glycosylation, O-GlcNAc, in metazoans. This modification is dynamic and responsive to numerous stimuli and conditions, suggesting an important role in many regulatory pathways. Moreover, the O-GlcNAc modification seems to compete with phosphorylation for sites of attachment, indicating a reciprocal relationship with phosph

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Techniques in Protein Methylation,hanging the primary sequence of a peptide so that it can encode more information. This common posttranslational modification is implicated in the regulation of a variety of processes including protein trafficking, transcription and protein-protein interactions. In this chapter, we will use the argin

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