Titlebook: SiRNA Delivery Methods; Methods and Protocol Kato Shum,John Rossi Book 2016 Springer Science+Business Media New York 2016 RNA interference.

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Aptamer-MiRNA Conjugates for Cancer Cell-Targeted Delivery,ls for the selective accumulation of miRNA to target tumors, their intracellular uptake, processing, and functional silencing of target genes. In this chapter, we discuss the direct conjugation of miRNAs to aptamers against transmembrane receptors as innovative experimental approach for their selective delivery to cancer cells.

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Stable Delivery of CCR5-Directed shRNA into Human Primary Peripheral Blood Mononuclear Cells and He in mammalian cells. Here, we describe our protocols for the development of shRNA against a major HIV co-receptor/chemokine receptor CCR5 and the use of lentiviral vectors for stable shRNA delivery and expression in primary human PBMC and HSPC.

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Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates,olymers using a controlled polymerization method, as well as the preparation of the resulting targeted SiRNA polymer conjugates. In addition, detailed methods are provided for the characterization of the biodegradable poly(peptides) as well as the polymer conjugate that ensues.

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Synthesis and Conjugation of Small Interfering Ribonucleic Neutral SiRNNs,hold significant promise as a therapeutic modality. However, the size and high negative charge of siRNAs render them unable to enter cells without assistance from a delivery agent. Most current methods of siRNA delivery rely on encasing siRNA molecules in large nanoparticles or cationic liposomes. H

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Liver-Targeted SiRNA Delivery Using Biodegradable Poly(amide) Polymer Conjugates,gh many literature examples of polymer conjugate-based SiRNA delivery systems exist, the protocols described herein represent a robust and facile way of screening any poly(amine)-based polymer system for SiRNA delivery. In this chapter, we describe the synthetic methods used to prepare poly(amide) p

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