Titlebook: Serum/Plasma Proteomics; Methods and Protocol David W. Greening,Richard J. Simpson Book 2017Latest edition Springer Science+Business Media,

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A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Studiesate contains factor VIII (antihemophilic factor), von Willebrand factor (vWF), fibrinogen, factor XIII, fibronectin, and small amounts of other plasma proteins. Clinical grade preparations of cryoprecipitate are mainly used to treat fibrinogen deficiency caused by acute bleeding or functional abnorm

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Preparation of Platelet Concentrates for Research and Transfusion Purposes host defense, thrombosis, inflammation, and tumor metastasis. Activation of platelets is crucial for platelet function that includes a complex interplay of adhesion, signaling molecules, and release of bioactive factors. Transfusion of platelet concentrates is an important treatment component for t

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Bead-Based and Multiplexed Immunoassays for Protein Profiling via Sequential Affinity Capturein solution to enable a direct readout on the single binder assay format or detected by matched pairs of capture and detection antibodies in dual binder assay format, also known as sandwich assays. Aiming to benefit from the flexibility and capacity offered by single binder assay readout and the spe

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Low-Molecular-Weight Plasma Proteome Analysis Using Top-Down Mass Spectrometryome (<30 kDa) has been shown to contain a rich source of diagnostic biomarkers. Here we describe a protocol for top-down proteomic analysis to identify and characterize the LMW proteoforms present in four types of human plasma samples without immunoaffinity depletion and with depletion of the top tw