Titlebook: Sertoli Cells; Methods and Protocol Marco G. Alves,Pedro F. Oliveira Book 2018 Springer Science+Business Media, LLC 2018 Andrology.Cell cul

Morsel

Marco G. Alves,Pedro F. OliveiraIncludes cutting-edge techniques for the study and use of Sertoli cells.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts

Urologist

cartilage

Sertoli Cells978-1-4939-7698-0Series ISSN 1064-3745 Series E-ISSN 1940-6029

Pulmonary-Veins

Book 2018e need for standardized protocols for their .in vitro. culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond t

工作

不怕任性

DOTE

Sertoli Cell Preparation for Co-immunoprecipitation,f your study, the technique used to lyse and consequently obtain protein extracts from Sertoli cells must be considered. In this chapter, we will focus on how to obtain a protein Sertoli cell extract suitable for co-immunoprecipitation technique.

AUGER

Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscy and its specificities in the seminiferous tubules will be briefly reviewed, guidelines of analysis of main phagocytosis steps by confocal and transmission electron microscopy will be described, and a simplified method to assess phagocytosis rate in routine experiments will be given.

adumbrate

Assessing Sertoli Cell Metabolic Activity,cs in the research on Sertoli cell metabolism. Main principles and techniques of cell medium, cellular extracts, and intact cells are explained, as well as some classical experiments that can give complementary information on the Sertoli cell metabolism.

aerobic

A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sepididymal ducts. Here, we describe a stopped-flow light-scattering methodology to easily assess the Sertoli cell osmotic water permeability. The devised methodology is well suited for studying how the Sertoli cell’s permeability changes following exogenous stimuli.