Titlebook: Sepsis; Methods and Protocol Wendy E. Walker Book 2021 Springer Science+Business Media, LLC, part of Springer Nature 2021 Biomarkers.Pathop

空气传播

A Mouse Model of , Pneumonia,perimental mouse model of respiratory infection by gram-negative . and analyses of leukocyte trafficking in the lungs. The reader will learn two methods to induce respiratory infection in mice that differ in whether the initial bolus is targeted within a specific lobe of the lung. We then describe a

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itinerary

Infection of Mice as a Model of Sepsis,he systemic production of a storm of cytokines is common in all etiologies of sepsis. While the complexity of this uncontrolled cascade is difficult to replicate using single molecule agonist, for example, lipopolysaccharide (LPS), several whole organism models can stimulate this cytokine storm. Her

Invigorate

A Mouse Model of Necrotizing Enterocolitis,terized by impairment of the intestinal barrier resulting in intestinal necrosis and multisystem organ failure. Animal models of NEC have contributed significantly to a better understanding of the underlying molecular mechanisms of the disease and facilitated the exploration of potential new therape

Excitotoxin

A Murine Model of Full-Thickness Scald Burn Injury with Subsequent Wound and Systemic Bacterial Infribe a murine model of severe burn injury followed by subsequent postburn infection, both local and systemic, that leads to sepsis. A detailed description of the full-thickness scald burn procedure is provided, followed by description of infection with two common burn-associated nosocomial pathogens

Platelet

Mouse Intensive Care Unit (MICU),ion might be the discrepancy between preclinical models and the clinical reality: nonresuscitated young healthy rodents in contrast to elderly comorbid patients in an intensive care unit. We introduce the mouse intensive care unit (MICU) as a concept to address the lack of resuscitation in preclinic

BLINK

Creation of BLT Humanized Mice for Sepsis Studies,t is ethically preferable to use mice for research over higher sentient species, when scientifically appropriate. Mice are also advantageous for research due to their small size, modest housing needs, the availability of genetically modified strains, and the broad range of reagents available for sci

Camouflage

Scoring Sepsis Severity in Mice,es in animal models of sepsis. Mice are used in the majority of preclinical sepsis studies. We describe a murine sepsis score that evaluates seven clinical variables in an experimental mouse model of polymicrobial sepsis.

镀金

Identification of ILC2 in the Lung Using Flow Cytometry,ity and adaptive immunity (Germain and Huang. Curr Opin Immunol 56:76–81, 2019; Krabbendam et al. Immunol Rev 286:74–85, 2018; Mindt et al. Front Immunol 9:840, 2018) . Recent studies reveal that ILC2 play critical roles in inflammation-associated lung injury during sepsis (Lai et al. Cell Death Dis

grovel

Measurement of Intestinal Permeability During Sepsis,termination of barrier dysfunction under conditions of health and disease. Fluorescence-conjugated dyes such as fluorescein isothiocyanate-4 kDa dextran (FD4) have been commonly used for evaluating hyperpermeability. Here we describe a common method to measure gut permeability in vivo, following gav