Titlebook: Salamanders in Regeneration Research; Methods and Protocol Anoop Kumar,András Simon Book 2015 Springer Science+Business Media New York 2015

Nucleate

Jean-François Denis,Fadi Sader,Patrizia Ferretti,Stéphane Roy Ph.D.e. This phenomenon is particularly acute in anaerobic sulfate-rich environments. It is therefore important to reliably assess the inherent susceptibility of fuels to anaerobic biodegradation in marine waters as well as provide a measure of the impact of this metabolism on the integrity of steel. Suc

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Chromatic

Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryosc larva and the aneurogenic limb (ANL) develops independently without innervation. Paradoxically, the limb in an ANL is capable of regeneration in a nerve-independent manner. Here, we describe a detailed method for the generation of ANL in the spotted salamander, ., for regeneration studies.

GRUEL

Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemasion and maintenance of cell lines from adult post-metamorphic salamanders and in particular cells derived from normal and regenerating limbs. We also describe a protocol for nucleofecting newt cells that can be used both to investigate the gene function in short-term studies and to establish stable cell lines.

Gene408

Husbandry of Spanish Ribbed Newts (,) The methods are outlined to manage housing, feeding, handling, captive breeding, health monitoring, and euthanasia in this species under laboratory conditions. With minor changes, these protocols can also be applied to other species of urodele amphibians commonly used in laboratory research.

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NOVA

Culture and Transfection of Axolotl Cellsnd in many situations these experiments help understand what is happening in the whole organism. In this chapter, we describe techniques for the culture and genetic manipulation of an established cell line from axolotl, a species widely used for studying epimorphic regeneration.

不可思议

Isolation and Culture of Neurospheres from the Adult Newt Brainting and culturing of NSCs from the newt brain both during self-renewing and differentiating conditions. Newt NSC culture provides a useful tool for functional studies of NSC fate with the potential of resulting in novel regenerative strategies.

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