Titlebook: SV40 Protocols; Leda Raptis Book 2001 Springer Science+Business Media New York 2001

Commodious

BROOK

哄骗

Colonnade

muscle-fibers

Propagation and Assay of BK Virus, England in 1971 (.). Seroprevalence studies have shown that BK infection is widespread in children throughout the world, and the virus thereafter persists latently in the kidney for life. Immunosuppression can lead to reactivation of the virus and prolonged viruria (.). Although in immunocompetent

有助于

单挑

Methods for Studying Interactions Between Simian Virus 40 T-Antigen and the Viral Origin of Replicamajor advantage of this system is that, with the exception of one viral protein, termed T-antigen (T-ag), all of the proteins required for DNA replication are supplied by cells that are permissive for SV40 replication (.). Because the virus depends on host proteins for replication (.), the SV40 syst

馆长

Quantitation and Structural Analysis of SV40 RNAs, the late unit, which is expressed at a significant level only after the onset of viral DNA replication (.). The early genes encode the viral regulatory proteins, small, and large T-antigens. These proteins play roles in replication of the viral genome and transcriptional transactivation of the late

corpuscle

时代

Production of SV40 Proteins in Insect Cells and In Vitro Packaging of Virions and Pseudovirions,tors. Although prokaryotic expression systems provide higher yields and are technically simpler to use, obtaining biologically active eukaryotic proteins in these systems can be problematic. Posttranslational modifications such as glycosylation and phosphorylation do not occur in prokaryotic systems