Titlebook: SILAC; Methods and Protocol Jose L. Luque-Garcia Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to Spri

ALLAY

Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Qumatic detection at near physiological conditions. The addition of a quantitative proteomic method, like SILAC metabolic labeling, allows the elimination of non-specifically bound contaminants which greatly increases the confidence of the identified interaction partners. Compared to eukaryotic cells,

macrophage

pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles,nvironmental cues can modify EV loading with proteins derived from the plasma membrane via endocytosis, obtained from the preexisting cytosolic pool via active sorting, or packaging with newly synthesized proteins drawn from trans-golgi networks. Given the major impact these pathways exert on EV con

TERRA

Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC),, DNA repair, transcriptional control, and chromatin remodeling. Interestingly, various stress conditions such as heat shock, oxidative stress, and ischemia promote global changes in sumoylation in different cells or tissues. However, due to limitations in either abundance or steady state sumoylatio

救护车

A Super-SILAC Approach for Profiling Histone Posttranslational Modifications,physiological and pathological processes. During the last decade, mass spectrometry (MS) has emerged as the most accurate and versatile tool to quantitate histone PTMs. Stable-isotope labeling by amino acids in cell culture (SILAC) is an MS-based quantitation strategy involving metabolic labeling of

Formidable

SILAC-Based Quantitative Phosphoproteomics in Yeast, evaluation of phosphopeptide enrichment, and sample preparation and analysis by high-resolution LC-MS/M, identification of phosphosites, and quantification methods are described..We report methods for the application of double SILAC to yeast using a combination of labeled lysine and labeled arginin

Tempor

Phosphotyrosine Profiling Using SILAC, phosphotyrosine profiling can reveal tyrosine kinase signaling activity in cells. Using quantitative proteomics strategies such as stable isotope labeling with amino acids in cell culture (SILAC) allows comparison of tyrosine kinase signaling activity across two to –three different conditions. In t

试验

Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins,n combination with stable isotope labeling by amino acids in cell culture (SILAC), for the proteome-wide profiling of geranyl pyrophosphate (GPP)-binding proteins. After labeling using a desthiobiotin-GPP acyl phosphate probe, desthiobiotin-conjugated peptides of GPP-binding proteins could be enrich

反对

Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactivesis, membrane trafficking, and storage of Ca.. Therefore, global profiling of ER-associated proteins should be invaluable for understanding these biological processes. However, the difficulty of isolating the intact ER hampered proteome-wide analysis of ER proteins. This chapter describes a chemopr

Addictive

Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC,s in different conditions simultaneously. In recent years, 3D cell growth culture conditions have been developed to establish intestinal organoids from isolated crypts, which mimic the intestine’s cell composition and organization. Organoids, isolated from normal or diseased tissues, can be used to

Polydipsia

Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Culturel culture (SILAC) requires nearly complete metabolic labeling of proteins and therefore is difficult to apply to cultured primary neurons, which do not divide in culture. In a multiplex SILAC strategy, two different sets of heavy amino acids are used for labeling cells for the different experimental