Titlebook: Rift Valley Fever Virus; Methods and Protocol Pierre-Yves Lozach Book 2024 The Editor(s) (if applicable) and The Author(s), under exclusive

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Fanny Ott,Märit-Runa Jönsson,Kay Grünewald,Jan Hellert

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Expression and Purification of RVFV Glycoprotein C for Structural Studies Using Baculovirus Expressn. In this chapter, we describe a simplified and scalable protocol facilitating the generation of high-quality, high-titer baculovirus virus for expression and purification of RVFV G., utilizing the baculovirus-mediated expression system in insect cells.

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Nucleotide Resolution Mapping of Rift Valley Fever Virus Nucleoprotein-Genome RNA Interactions,inking of direct protein-RNA interactions by UV irradiation. Following sample lysis, a selective isolation of N in complex with its RNA targets is achieved by immunoprecipitation. Then, N-RNA complexes are separated by SDS-PAGE, and after membrane transfer, RNA is isolated and subjected to library p