Titlebook: Rice Genome Engineering and Gene Editing; Methods and Protocol Anindya Bandyopadhyay,Roger Thilmony Book 2021 Springer Science+Business Med

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Cumbersome

Genotype-Independent Regeneration and Transformation Protocol for Rice Cultivarsistic transformation protocol for rice varieties. Putative transgenic rice lines were confirmed by PCR analysis, DNA sequencing, and Southern analysis. The transformation protocol reported here is relatively simple and consistent and can be exploited in future biotechnological investigations particularly for gene transformation studies.

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incisive

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Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencingols. Whole-genome single nucleotide variations (SNVs) and indels are detected by LoFreq., Mutect2, VarScan2, and Pindel. Last, SNVs and indels are compared with in silico off-target sites using Cas-OFFinder.

STING

Efficient Genome Editing in Rice Protoplasts Using CRISPR/CAS9 Construct in protoplasts prepared from leaf tissues of 100, 9–11-day-old seedlings. CRISPR/CAS9 construct effectiveness can be clearly established within less than a week. We provide here a full protocol, from designing sgRNA to mutation analysis.

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Improving a Quantitative Trait in Rice by Multigene Editing with CRISPR-Cas9practiced including Golden Gate assembly. This chapter provides a detailed protocol for the construction of a T-DNA binary vector for expressing Cas9 and three sgRNAs for simultaneous targeting of three QTL genes for improving seed trait in rice.

Intellectual

1064-3745 format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoidi978-1-4939-5729-3978-1-60761-535-4Series ISSN 1064-3745 Series E-ISSN 1940-6029

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The Witness-People Myth and Its Alternatives,istians. The special stature of the Jewish people was not lost in the angry disputes of the first century or the fateful parting of the ways in the second. Emerging “Christianity” of the first century . defined itself primarily in relation to mainstream Judaism and its interpretation of Jewish Scrip