Titlebook: Reversible Protein Phosphorylation in Cell Regulation; R. L. Khandelwal,J. H. Wang Book 1993 Springer Science+Business Media Dordrecht 199

Servile

Chicken smooth muscle myosin light chain kinase is acetylated on its NH2-terminal methionine 1990). The calculated Mr is 107,534 whereas the estimate by SDS-PAGE is approximately 130,000. Gibson and Higgins (DNA Sequence (in press)) have recently reported the possibility of errors in the cDNA sequence for non-muscle MLCK and that the NH.-terminus of both it and smMLCK may extend beyond the

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Mutagenesis of the catalytic subunit of rabbit muscle protein phosphatase-1eptible to inactivation by sulfhydryl reagents, we have mutagenized the six cysteine resid ues conserved between these two enzymes to serines. The six mutants were purified to near homogeneity by affinity chromatography on inhibitor-2-Sepharose and characterized. All six exhibited enzymatic activity

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Serine phosphorylation of protein tyrosine phosphatase (PTP1B) in HeLa cells in response to analogueparticulate fraction of mammalian cells and can be solubilized as a complex of 150 kDa by extraction with non-ionic detergents. Previous work from this laboratory implicated phosphorylation of serine/ threonine residues in the regulation of this PTP. Activity was several-fold higher in cells treated

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FORGO

Protein tyrosine phosphatase activity in ,d in fractions following gel filtration column chromatography. Over 90% of the activity was soluble in a low salt extraction buffer in both phases of growth. Several peaks of activity were resolved following gel filtration of the crude extracts indicating that multiple tyrosine phosphatases are pres

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The phosphorylation of stathmin by MAP kinasetiation and other cell functions, was found to be phosphorylated rapidly and stoichio-metrically by mitogen-activated protein (MAP) kinase .. Ser-25 was identified as the major site and Ser-38 as a minor site of phosphorylation, while the p42 and p44 isoforms of MAP kinase were the only significant

尾巴

Networking with mitogen-activated protein kinasesular signaling pathways of many diverse cytokines. Recent studies have extended this protein kinase cascade by identifying the major activator of vertebrate MAP kinases as a serine/threonine/tyrosine-protein kinase called MEK, which is related to yeast mating factor-regulated protein kinases encoded

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