Titlebook: Retinal Development; Methods and Protocol Chai-An Mao Book 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020 central

MEAN

Identification and Characterization of Cis-Regulatory Elements for Photoreceptor-Type-Specific Trand blue (RGB) cone-specific transcription requires its enhancer, a member of the rainbow enhancer family. While exemplified with an RGB-cone-specific gene, this protocol is general and can be used to identify the core promoters and enhancers of other protein-coding genes.

Creditee

Live Imaging of Mouse Retinal Slices,g slice cultures that are suitable for capturing discrete cellular events during retinal development and differentiation. This is a significant improvement upon current methods, as it is more amenable to a wider array of imaging systems and does not compromise resolution of retinal cross-sectional area.

允许

Transposon-Mediated Stable Suppression of Gene Expression in the Developing Chick Retina,e using the Tol2 transposon system. We show that this methodology can induce potent and stable suppression of gene expression. This technique therefore provides a rapid and robust loss-of-function approach for studies of gene function in the developing retina.

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群居动物

effrontery

deciduous

勤劳

abduction

Three-Dimensional Culture of Mouse Eyecups, intrinsic and extrinsic factors, but the molecular mechanism underlying the process is partially understood. Functional studies using engineered mice provide tremendous insight into the mechanisms of retinal cell differentiation, but in utero embryogenesis prevents manipulations of mouse embryonic

破布