Titlebook: Restriction Landmark Genomic Scanning (RLGS); Yoshihide Hayashizaki,Sachihiko Watanabe Book 1997 Springer Japan 1997 DNA.cancer.chromosome

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RLCS, Restriction Landmark cDNA Scanning,sion; it is simple, sensitive, and time-saving. However, PCR-mediated amplification with arbitrary primers requires delicate control, depending on the primers used [5,6], and cDNA probes thus obtained often give false positive signals on Northern blot analysis [7,8]. Furthermore, the differential di

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Protocols for RLGS Gel Production,he recognition site of enzyme E.. In addition, the reaction buffers used in each process should be changed, accompanied by variation of the enzyme. Here, protocols for two good representative enzyme combinations, combination 1 (.I-.II-.I) and combination 2 (.I-.RV-.I), are shown.

Free-Radical

RLGS Spot Cloning,direct method for recovering landmark spots or sites is also essential for transferring the information to other laboratories and for identifying the gene functions that may be associated with these sites. The following points must be considered in the recovery of sequences associated with an RLGS spot.

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https://doi.org/10.1007/978-4-431-67953-0DNA; cancer; chromosome; cloning; genome; linkage analysis; mapping; tissue

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Yoshihide Hayashizaki,Sachihiko WatanabeThe first laboratory manual with detailed techniques on RLGS, a rapid method for genome-wide scanning with broad applications in biological and medical research.