Titlebook: Restriction Endonucleases; Alfred M. Pingoud Book 2004 Springer-Verlag Berlin Heidelberg 2004 ATP.DNA.DNA-Erkennung.DNA-Spaltung.Restrikti

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Protein Engineering of Restriction Enzymes,icity is one of the scientific goals in studying these enzymes (.). This review focuses on protein engineering regarding Type II restriction enzymes. They comprise the vast majority of the about 3600 entities listed in REBASE today (.), although the number of those studied in detail is far smaller.

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0933-1891 ucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known repre

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Book 2004gainst foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic seque

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Restriction-Modification Systems as Minimal Forms of Life, Restriction enzymes will cleave incoming DNA if it has not been modified by a cognate or another appropriate methyltransferase (Fig. 1B). Consequently, it is widely believed that restriction-modification systems have been maintained by bacteria because they serve to defend the cells from infection by viral, plasmid, and other foreign DNAs(.).

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Restriction Endonucleases: Structure of the Conserved Catalytic Core and the Role of Metal Ions in this Vol.) cleave specifically within or close to their recognition sites, and do not require ATP hydrolysis for their nucleolytic activity. DNA cleavage by these enzymes can result in DNA with either 5′ or 3′ overhangs or blunt ends.

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The Role of Water in the EcoRI-DNA Binding,TTC, with an association equilibrium constant K.∼10. M. and to a completely nonspecific sequence with K. ∼10.M.. A change of even a single base pair is sufficient to decrease the binding constant at least by 10., bringing it within a factor ∼10 or less of nonspecific binding (.).

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