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Titlebook: Resin Microscopy and On-Section Immunocytochemistry; Geoffrey R. Newman,Jan A. Hobot Book 19931st edition Springer-Verlag Berlin Heidelber

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书目名称Resin Microscopy and On-Section Immunocytochemistry
编辑Geoffrey R. Newman,Jan A. Hobot
视频video
丛书名称Springer Lab Manuals
图书封面Titlebook: Resin Microscopy and On-Section Immunocytochemistry;  Geoffrey R. Newman,Jan A. Hobot Book 19931st edition Springer-Verlag Berlin Heidelber
描述For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.
出版日期Book 19931st edition
关键词Colloidal Gold; Harz-Einbettung; Immunomicroscopy; Immunomikroskopie; Kolloidales Gold; Resin Embedding; m
版次1
doihttps://doi.org/10.1007/978-3-642-97481-6
isbn_ebook978-3-642-97481-6
copyrightSpringer-Verlag Berlin Heidelberg 1993
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Immunolabelling Protocols for Resin SectionsThe protocols for immunolabelling have been set out in three main parts:
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Springer-Verlag Berlin Heidelberg 1993
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https://doi.org/10.1007/978-3-642-97481-6Colloidal Gold; Harz-Einbettung; Immunomicroscopy; Immunomikroskopie; Kolloidales Gold; Resin Embedding; m
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Cryotechniques. The prerequisite for cryotechniques is for biological tissue to be rapidly frozen prior to any processing steps. Processing can then be in to resin (cryosubstitution, Sect. 4.3; freeze-drying, Sect. 4.6), or the frozen tissue can be sectioned directly (cryoultramicrotomy, Sect. 4.9).
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Methods for Resin Polymerisationalysts (Glauert, 1975). However, considerably longer times are needed for the tissue to be present in an organic monomer (Sect. 1.3.1). These methods are not used routinely and references to them in the literature are scarce.
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