Titlebook: Regulatory T-Cells; Methods and Protocol Masahiro Ono Book 2023 The Editor(s) (if applicable) and The Author(s), under exclusive license to

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Book 2023ders through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful .Methods in Molecular Biology .series, each chapter includes an introduction to t

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1064-3745 ation advice from the experts.This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods

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Adoptive Transfer and Bone Marrow Chimera Models to Analyze Treg Function and Differentiation. Treg function can be quantitatively measured by analyzing the expansion of conventional CD4 T cells and their differentiation into helper cells. The quantitative measure of Treg differentiation is addressed by analyzing the number and phenotype of Foxp3-expressing cells. The use of congenic markers is instrumental for these approaches.

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