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Titlebook: Regulatory B Cells; Methods and Protocol Gaetano Vitale,Francesca Mion Book 2014 Springer Science+Business Media New York 2014 Anti-inflamm

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发表于 2025-3-21 17:47:54 | 显示全部楼层 |阅读模式
书目名称Regulatory B Cells
副标题Methods and Protocol
编辑Gaetano Vitale,Francesca Mion
视频video
概述Includes cutting-edge methods and protocols involving B cells with suppressive functions and their immunological properties.Provides step-by-step detail essential for reproducible results.Contains key
丛书名称Methods in Molecular Biology
图书封面Titlebook: Regulatory B Cells; Methods and Protocol Gaetano Vitale,Francesca Mion Book 2014 Springer Science+Business Media New York 2014 Anti-inflamm
描述.Regulatory B Cells: Methods and Protocols. present the current experimental set-ups and methodologies useful for the identification and characterization of B cells with suppressive functions and for the study of their biological and immunological properties. Organized into four sections, this detailed volume covers the basic methods for the isolation and immunophenotypical characterization of specific B cell subsets from mouse and human tissues, methods for the investigation of the mechanisms of immune suppression operated by B cells, several experimental approaches for the ex vivo generation/expansion of IL-10-producing B cells, as well as procedures for the study of the immune suppressive function of B cells in specific pathological settings. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls..Practical and authoritative, .Regulatory B Cells: Methods and Protocols. serves as an ideal guide for immunologists as well as for cell and molecu
出版日期Book 2014
关键词Anti-inflammatory cytokines; Breg; IL-10; Immune regulation; Immune suppression; Pathophysiology; Suppress
版次1
doihttps://doi.org/10.1007/978-1-4939-1161-5
isbn_softcover978-1-4939-4677-8
isbn_ebook978-1-4939-1161-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2014
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Purification and Immunophenotypic Characterization of Murine MZ and T2-MZP Cells several B-cell subsets representing various maturation stages and/or cell functions. The spleen is a complex lymphoid organ organized in two main structures with different functions: the red and white pulp. The red pulp is flowed with blood while the white pulp is organized in primary follicles, wi
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Purification and Immune Phenotyping of B-1 Cells from Body Cavities of Mice. While B-1-cell frequencies in secondary lymphoid tissues are low, relative high frequencies are found within peritoneal and pleural cavities of mice, including both CD5. B-1a and CD5. B-1b cells. They represent reservoirs of B-1 cells that can be activated for migration to lymphoid tissues to secr
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Purification and Immunophenotypic Characterization of Murine B10 B Cellsion with a combination of B cell mitogen and chemical stimulants. Although B10 cells only constitute 1–2 % of splenic CD19. B cells, they play important roles in controlling T cell-mediated immune responses in an antigen-specific and IL-10-dependent manner. The regulatory effects of B10 cells have b
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Characterization and Activity of Fas Ligand Producing CD5+ B Cellssive cytokines, and expression of death-inducing ligands. A role for Fas ligand (FasL)-expressing “killer” B cells in regulating T helper cell survival and chronic inflammation has been demonstrated in animal models of schistosome worm infection, asthma, and autoimmune arthritis. Interestingly, a po
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