Titlebook: Regulatory B Cells; Methods and Protocol Francesca Mion,Silvia Tonon Book 2021Latest edition Springer Science+Business Media, LLC, part of

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Detection of IL-10 in Murine B Cells: In Vitro and In Vivo Techniquesfor the detection of IL-10 production in B cells. In this chapter, we describe several commonly used methods for the effective detection of IL-10 in B cells at both mRNA and protein levels, including quantitative PCR analysis, intracellular staining of IL-10 in live B cells by flow cytometry, ELISA