Titlebook: Recombinant and In Vitro RNA Synthesis; Methods and Protocol Graeme L. Conn Book 2012 Springer Science+Business Media, LLC 2012 RNA purific

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Christopher J. Kershaw,Raymond T. O’Keefer und beschreiben aus welchen fünf zentralen Fragmenten sich diese zusammensetzt. Anschließend stellen sie ihren vielfach in der Praxis erprobten Prozess vor, der Kultur sichtbar, verständlich und damit für all978-3-658-42768-9978-3-658-42769-6

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General Protocols for Preparation of Plasmid DNA Template, RNA In Vitro Transcription, and RNA Purier the last three decades. The numerous approaches available today to study RNA structure and function vary widely in their demands on the quality and quantity of material needed. These range for example from a few micrograms in biochemical assays, RNA structure probing or RNA folding studies using

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Preparation of Short RNA by In Vitro Transcription,t of specific RNA sufficient for structural studies in a more economical way than chemical synthesis. Here, we provided protocols optimized for the synthesis of very short RNA of 10–20 nucleotides in length by in vitro transcription. These RNA oligomers can be purified conveniently by gel filtration

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