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Titlebook: Recombinant Proteins from Plants; Charles Cunningham,Andrew J. R. Porter Book 1998 Humana Press 1998

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Transient Gene Expression in Plant Protoplasts,d proteins prior to stable transformation. Although stably transformed plant material may be preferable in many instances, transformation and regeneration of plants is time-consuming, tedious, and requires plenty of space. Transient expression techniques enable measurement of gene expression, as wel
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Stability of Recombinant Proteins in Plants,ndogenous metabolic reactions, but also with the degradation of abnormal or exogenous (“foreign”) proteins (.). Despite the fundamental importance of proteases in cells, studies on these enzymes, for those involved in gene-expression technology, are devised to develop a means of avoiding or minimizi
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Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Prote fundamental research. Reporter proteins can be used to characterize promoter sequences and other .-acting sequences; the overproduction of proteins in plants can help to elucidate their function. Second, the synthesis of heterologous proteins in plants can have biotechnological applications. The in
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Use of the GUS Reporter Gene,n of promoters, through the transformation of plants with a promoterless reporter gene construct. Activation of the reporter gene will then only occur if the gene is inserted adjacent to a native promoter (.).
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