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Titlebook: Recombinant Proteins from Plants; Charles Cunningham,Andrew J. R. Porter Book 1998 Humana Press 1998

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Recombinant Proteins from Plants978-1-60327-260-5Series ISSN 1940-6061 Series E-ISSN 1940-607X
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Production of Recombinant Antibodies in Plant Suspension Cultures,ess RAbs against almost any given antigen, and to fine-tune these molecules with respect to improved performance. Furthermore, incorporation of affinity tags has simplified the purification of heterologously expressed recombinant proteins (.,.).
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Screening for Transgenic Lines with Stable and Suitable Accumulation Levels of a Heterologous Proteurposes, the expression profile and accumulation level of the heterologous protein in plants should be stable, reproducible, and suitable. This chapter deals with the following problems∶ Most primary transformants show relatively low expression levels of the transgene (.); and expression levels are not always stably transmitted to the progeny.
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Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants,nt tissues are generally recognized as safe, the probability of health risks because of contamination with potential human pathogens and toxins in recombinant products derived from plants is minimized. Furthermore, plants have glycosylation machinery like other eukaryotic systems (.).
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Quantification of Heterologous Protein Levels in Transgenic Plants by ELISA,obic attractive forces (.). The high specificity of this interaction enables an antibody to recognize its antigen even in the presence of a huge amount of contaminating antigens. This makes antibodies indispensable tools in a wide range of disciplines, including molecular biology.
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1940-6061 ing applied to the exploitation of plants as novel expression systems for the p- duction and overproduction of heterologous and native proteins. Transgenic plant technology is currently used in three broad areas: the expression of - combinant proteins to improve crop quality by increasing disease/pe
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Characterization and Applications of Plant-Derived Recombinant Antibodies,s), endoplasmic reticulum, chloroplasts, and the intercellular space (full-size, scFvs, and single-domain antibodies) for various applications (.–.). These results indicate the flexibility of the plant system for expression of rAbs, or fragments thereof, in various plant cell compartments.
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