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Titlebook: Recombinant Proteins from Plants; Methods and Protocol Jacqueline MacDonald,Igor Kolotilin,Rima Menassa Book 2016Latest edition Springer Sc

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发表于 2025-3-21 16:41:39 | 显示全部楼层 |阅读模式
书目名称Recombinant Proteins from Plants
副标题Methods and Protocol
编辑Jacqueline MacDonald,Igor Kolotilin,Rima Menassa
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: Recombinant Proteins from Plants; Methods and Protocol Jacqueline MacDonald,Igor Kolotilin,Rima Menassa Book 2016Latest edition Springer Sc
描述.This volume provides up-to-date scientific achievements from the world’s top researchers. .Recombinant. .Proteins from Plants: Methods and Protocols, Second Edition. guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and practical., .Recombinant. .Proteins from Plants: Methods and Protocols, Second Edition. is an ideal reference for those who are interested in plant molecular biology and molecular farming..
出版日期Book 2016Latest edition
关键词cloning; molecular farming; plant expression systems; plants; recombinant proteins; transgenic plants
版次2
doihttps://doi.org/10.1007/978-1-4939-3289-4
isbn_softcover978-1-4939-5004-1
isbn_ebook978-1-4939-3289-4Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
The information of publication is updating

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Production of Recombinant Cholera Toxin B Subunit in , Using GENEWARE, Tobacco Mosaic Virus Vectored proteins. CTBp is purified to >95 % homogeneity by two-step chromatography using immobilized metal affinity and ceramic hydroxyapatite resins. This procedure yields on average 400 mg of low-endotoxin CTBp from 1 kg of fresh leaf material.
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Chloroplast-Based Expression of Recombinant Proteins by Gateway® Cloning Technology the past decade. Several improvements of the technology have turned plant plastids into robust and dependable expression platforms for multiple high value compounds. In this chapter, we describe our current methodology based on Gateway. recombinant cloning, which we have adapted for plastid transfo
发表于 2025-3-22 12:38:30 | 显示全部楼层
Multigene Engineering in Rice Using High-Capacity , BIBAC Vectorsnts via .-mediated transformation. Here, we describe an optimized protocol for transformation of japonica rice (. L.) using this system. Calli derived from mature embryos are transformed using . strain LBA4404 that carries the BIBAC vector and the super-virulent helper plasmid pCH32. Transformed cal
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Virus-Derived Vectors for the Expression of Multiple Proteins in Plants The pEAQ vectors are a series of small binary vectors designed for controlled expression of multiple proteins in plants. To achieve high levels of expression, an expression system based on translational enhancement by the untranslated regions of RNA-2 from cowpea mosaic virus (CPMV), named CPMV-.,
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Transient Protein Expression by Agroinfiltration in Lettuceld and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to havi
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Production of Recombinant Proteins in the Chloroplast of the Green Alga ,rmation of . followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant pr
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Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semi modified crops. We provide a protocol for genetic transformation of two important crops, durum wheat and alfalfa, using a bacterial and a plant-derived selectable marker gene encoding mutated, gabaculine-insensitive glutamate 1-semialdehyde aminotransferase (GSA) enzymes. These methods can potentia
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