| 书目名称 | Recombinant Protein Production in Yeast | | 副标题 | Methods and Protocol | | 编辑 | Roslyn M. Bill | | 视频video | | | 概述 | Examines the process of preparation of expression vectors and disruption of yeast cells to enable to isolation of the recombinant protein.Provides step-by-step detail essential for reproducible result | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. .Recombinant Protein Production in Yeast: Methods and Protocols. examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful .Methods in Molecular Biology™. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. .Authoritative and practical,. Recombinant Protein Production in Yeast: Methods and Protocols,. see | | 出版日期 | Book 2012 | | 关键词 | bioreactor formats; enzymes; expression vectors; growth variables; high-yielding clones; medium compositi | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-61779-770-5 | | isbn_softcover | 978-1-4939-5871-9 | | isbn_ebook | 978-1-61779-770-5Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+business Media, LLC 2012 |
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发表于 2025-3-21 19:31:00
