| 书目名称 | Recombinant Protein Expression in Mammalian Cells | | 副标题 | Methods and Protocol | | 编辑 | David L. Hacker | | 视频video | | | 概述 | Includes cutting-edge methods and protocols for the study of recombinant protein expression.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .This detailed volume explores advances in vector design, DNA delivery, cell cultivation, host cell engineering, and bioprocess optimization within the study of recombinant protein expression in mammalian cells. The majority of the protocols employ either Chinese hamster ovary cells (CHO) or human embryonic kidney 293 cells (HEK293), the workhorses of the field, as the production host; however, the methods can be adapted to other mammalian hosts under the appropriate cell-specific conditions. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls...Authoritative and convenient, .Recombinant Protein Expression in Mammalian Cells: Methods and Protocols. aims to aid researchers in building on our knowledge of protein structure and function and to speed the discovery of new therapeutic proteins.. | | 出版日期 | Book 2018 | | 关键词 | Transient protein production; Recombinant cell lines; Chinese hamster ovary cells; Human embryonic kidn | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-8730-6 | | isbn_softcover | 978-1-4939-9377-2 | | isbn_ebook | 978-1-4939-8730-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2018 |
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发表于 2025-3-21 19:19:00
