Titlebook: Receptor-Receptor Interactions in the Central Nervous System; KJELL FUXE,Dasiel O. Borroto-Escuela Book 2018 Springer Science+Business Med

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Small Interference RNA Knockdown Rats in Behavioral Functions: GALR1/GALR2 Heteroreceptor in Anxietgenerated siRNA GALR1 and siRNA GALR2 rats to analyze the involvement of GALR1-GALR2 heteroreceptor complexes in the behavioral effects induced by GAL(1–15). In this chapter, we provide a protocol for using these animal models to investigate the role of heterodimers in vivo when a behavioral test is needed.

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,On the Study of D4R-MOR Receptor–Receptor Interaction in the Rat Caudate Putamen: Relevance on Morp as starting point: (1) quantitative receptor autoradiography (saturation assay); (2) agonist-stimulated [.S]GTPγS binding in autoradiography; and (3) immunohistochemistry. Together, they allow to explore functional changes in receptors signaling transduction, i.e., receptor recognition, G protein a

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,Use of Superfused Synaptosomes to Understand the Role of Receptor–Receptor Interactions as Integrat been extensively used to study the mechanism of neurotransmitter release in vitro because they preserve the biochemical, morphological, and electrophysiological properties of the synapse. This allows an unequivocal interpretation of results obtained under accurately specified experimental condition

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Detection of Fibroblast Growth Factor Receptor 1 (FGFR1) Transactivation by Muscarinic Acetylcholinransactivate tyrosine kinase receptors and their downstream intracellular signaling in absence of specific ligands. Here we describe our protocol for dissociating and maintaining hippocampal primary neurons in high- and low-density culture, followed by a description of methods employed to evaluate n