Titlebook: Receptor and Ion Channel Detection in the Brain; Rafael Lujan,Francisco Ciruela Book 2021Latest edition The Editor(s) (if applicable) and

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Study of GPCR Homo- and Heteroreceptor Complexes in Specific Neuronal Cell Populations Using the In and interactions between each other. Perturbation in specific heteroreceptor complexes and/or their balance/equilibrium with other heteroreceptor complexes and corresponding homoreceptor complexes is considered to have a role in pathogenic mechanisms, including drug addiction, depression, Parkinson.

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Amplified Luminescent Proximity Homogeneous Assay (Alpha)-Based Technique to Detect GPCR Oligomers iors (GPCRs) form the largest group of the receptors involved in cell signaling across the plasma membrane, thus being the main pharmacotherapeutic target. Importantly, GPCRs can interact between them, a phenomenon known as GPCR oligomerization. In recent years, the characterization of direct PPI bet

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Fluorescent In Situ Hybridization for Sensitive and Specific Labelinge probe analyses at a single-cell resolution. This protocol involves simultaneous and specific hybridization of hapten-labeled riboprobes for mRNAs, followed by probe visualization via immunohistochemical procedures and peroxidase-mediated precipitation of tyramide-linked fluorophores. This procedur

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细菌等

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Localization of GFP-Tagged Proteins Under the Electron Microscopes. Main advantages are its stability and its fluorescent activity independent on the binding of other ligands or proteins. The use of GFP-tagged proteins has found multiple applications in molecular biology such as lineage tracing assays, cell fusion assessment, and/or immunodetection among others.

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Tyramide Signal Amplification for Immunoelectron Microscopyy of any given antigen by catalyzing the deposit of labeled tyramide that is covalently bound near the target molecule, providing high-density signal. Current methods allow identification of any protein or RNA using antibodies or probes, respectively. This technique has also been validated for elect

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Pre-Embedding Methods for the Localization of Receptors and Ion Channelsnsights into the structure-function of neurotransmitter receptors and ion channels in the brain. These techniques involve immunoreactions with antibodies before embedding and ultrathin sectioning. As a result, they are very sensitive and show excellent resolution and good ultrastructural and antigen