Titlebook: Receptor Binding Techniques; Mary Keen Book 19991st edition Humana Press 1999

GROG

Hectic

Solubilized Muscarinic Acetylcholine Receptors from the Rat Myocardium: Pharmacological and Hydrodyntor can be solubilized without destroying its characteristic ability to bind specific ligands, it is possible to use the powerful tool of affinity chromatography in the purification procedure. Furthermore, investigation of the pharmacological and hydrodynamic characteristics of solubilized receptors

Left-Atrium

Radioligand Binding in Intact Cellsber of receptors and ligands (..–.). These experiments are generally performed under conditions that optimize the data obtained but are usually nonphysiological. It is difficult to compare the binding affinity obtained for a radioligand to cell membranes, in a low ionic strength buffer that contains

Cleave

歹徒

沉默

ANA

Autoradiography of Enzymes, Second Messenger Systems, and Ion Channelss have been studied using selective inhibitors, ion channels using naturally occurring toxins, and second messenger systems using inositol polyphosphates. Ligand binding complements immunohistochemistry and . hybridization by permitting pharmacological characterization and quantification of active s

里程碑

免除责任

Measurement of Agonist-Stimulated [35S]GTPγS Binding to Cell Membranesgonist binding to G-protein-coupled receptors (GPCRs), by monitoring the binding of [.S]GTPγS, a radiolabeled, hydrolysis-resistant analog of GTP (guanosine triphosphate), in the presence of unlabeled GDP (guanosine triphosphate).

garrulous

Characterization of Imidazoline Receptors by Radioligand BindingImidazoline receptors are divided into two main types, known as I.- and I.-receptors, and whereas I. receptors appear to play a role in control of blood pressure, I. receptor mediated functions are still unclear (.).