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Titlebook: Real-Time Analysis of Biomolecular Interactions; Applications of BIAC Kazuhiro Nagata,Hiroshi Handa Book 2000 Springer Japan 2000 biologica

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Peptide-Protein Interactionsclass I or MHC class II molecules complexed with appropriate peptides which are expressed on antigen presenting cells (APC) [1]. This activation is also significantly influenced by the T-cell coreceptor molecules, CD8 for MHC-I restricted and CD4 for MHC-II restricted cells, as well as other accesso
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Using BIACORE as Pots and Panse decided to prepare it by culturing hybridoma offered by a certain laboratory. But this hybridoma did not look very good and its growth rate was poor. Yet by persevering with cloning and culturing, it was eventually revived. So, 10µl of the cultured supernatant was diluted 10-fold and injected onto
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Principles of BIACOREamount of sample. For the interaction between biomolecules, the technique can measure not only the affinity at equilibrium (dissociation constant, .., or affinity constant, ..) but also the parameters that characterize the reaction rate, that is, the association rate constant .. and dissociation rate constant ...
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Principles of Surface Plasmon Resonanceefractive index, no radioactive, optical, or enzymatic labels are required. The technique can be used to study all kinds of molecules including proteins and protein conjugates, carbohydrates, lipids, nucleic acids, small molecules such as pharmaceuticals, metabolites and effectors, and even larger particles such as viruses and whole cells.
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