Titlebook: Rapid Cycle Real-Time PCR — Methods and Applications; Microbiology and Foo Udo Reischl,Carl Wittwer,Franklin Cockerill Book 2002 Springer-V

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Rapid Detection of Group B Streptococci Using the LightCycler Instrumentr, culture methods require up to 48 h to yield results and only predict 87% of women likely to be colonized by GBS at delivery [3]. A rapid, sensitive and specific test for detection of GBS directly from clinical specimens would allow for a simpler and more efficient prevention program.

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Genospecies-Specific Melting Temperature of the , PCR Product for the Detection of , sensu lato and ema migrans emerging around the tick-bite site. . sensu stricto, ., and . are the genospecies responsible for human Lyme borreliosis [2]. In Europe . and . are the most prevalent genospecies, whereas . sensu stricto is the only genospecies encountered in North America [2].

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tional PCR technology, Rapid Cycle Real-Time PCR is faster, has greater specificity, and is more easily adaptable for a variety of diagnostic tests, including qualitative, quantitative and mutation detection assays. This book provides general overviews of this technology for use in the clinical micr

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isms in human specimens and foodstuffs. All of these protocols have been developed, verified, and validated by experts in the field and should be of great interest for clinical microbiologists, pathologists, laboratory technologists as well as practicing physicians.978-3-642-48353-0978-3-642-48351-6