| 书目名称 | Rapid Cycle Real-Time PCR — Methods and Applications |
| 副标题 | Quantification |
| 编辑 | Carl Wittwer (Director of Flow Cytometry & New Tec |
| 视频video | http://file.papertrans.cn/822/821177/821177.mp4 |
| 图书封面 |  |
| 描述 | .Rapid cycle real-time PCR is a powerful technique for nucleic acid quantification and analysis that takes less than 30 minutes to complete. Fluorescence is automatically monitored each cycle and the amount of template quantified by advanced analytical methods, such as the second derivative maximum method. Immediately following rapid cycle PCR, melting curve analysis is performed to verify product purity with SYBR Green I and/or genotype with fluorescently-labeled hybridization probes (HybProbes or SimpleProbes). Rapid cycle real-time PCR is often cited as the most versatile, efficient method for nucleic acid quantification in research and climical studies. Molecular analysis has never been easier!. |
| 出版日期 | Book 2004 |
| 关键词 | DNA; LightCycler; PCR; Quantification; RNA; gene expression; transcription |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-642-18840-4 |
| isbn_softcover | 978-3-642-62317-2 |
| isbn_ebook | 978-3-642-18840-4 |
| copyright | Springer-Verlag Berlin Heidelberg 2004 |
|Archiver|手机版|小黑屋|
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