书目名称 | Rapid Cycle Real-Time PCR — Methods and Applications | 副标题 | Genetics and Oncolog | 编辑 | Wolfgang Dietmaier,Carl Wittwer,Natarajan Sivasubr | 视频video | | 图书封面 |  | 描述 | Rapid-Cycle Real-Time PCR is a powerful technique for nucleic acid amplification and analysis that often requires less than half an hour to perform. Samples are amplified by rapid-cycle PCR followed by immediate melting curve analysis in the same instrument. Melting curve analysis of PCR products with SYBR Green I often allows product identification without gel electrophoresis. Furthermore, in the presence of fluorescent hybridization probes, melting curves provide "dynamic dot blots" for fine sequence analysis, including single nucleotide polymorphisms (SNPs). The method is often cited as the most versatile, efficient method for nucleic acid analysis in research and diagnostics in the fields of genetics and oncology. Molecular diagnostics has never been easier! | 出版日期 | Book 2002 | 关键词 | DNA; TNF; biopsy; cancer; cytokine; diagnostics; gastric cancer; gene expression; genetics; genome; protein; sc | 版次 | 1 | doi | https://doi.org/10.1007/978-3-642-59397-0 | isbn_softcover | 978-3-642-63965-4 | isbn_ebook | 978-3-642-59397-0 | copyright | Springer-Verlag Berlin Heidelberg 2002 |
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