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Titlebook: Rapid Cycle Real-Time PCR; Methods and Applicat Stefan Meuer,Carl Wittwer,Kan-Ichi Nakagawara Book 2001 Springer-Verlag Berlin Heidelberg 2

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Use of TaqStart Antibody to Increase the Sensitivity of Herpesvirus Quantitative PCR on the LightCyce PCR on the LightCycler combines speed and accuracy. However, during PCR, non-specific amplification may compete with formation of specific products, leading to a reduced PCR efficiency and lower sensitivity. In this study, we demonstrate that an anti-Taq DNA polymerase antibody (TaqStart Antibody,
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High-Speed Detection of α1-Antitrypsin Deficiency Alleles Pi*S and Pi*Z on the LightCyclern damage the lung tissues, leading to pulmonary emphysema. More than 90 different alleles have been identified so far for the protease inhibitor 1 (.) gene. The three most important variants are type M (90% of population), type S (Pi*S) and type Z (Pi*Z). Homozygotes of type Z have a considerable re
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High-Speed Methylenetetrahydrofolate Reductase C → T 677 Mutation Detection on the LightCyclersm [1]. Genetic factors have been found to play a crucial role in disease development. Among these, mutations in the Factor V gene (FV Leiden) and in the prothrombin gene have been shown to account for a large number of thromboembolisms [2]. An additional independent risk factor is hyperhomocysteine
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Dual Color Detection of Splice Variants of the c-erbA α (Thyroid Hormone Receptor α) Geneere are two genes encoding for these receptors giving rise to four isoforms of the receptor (α1, α2, β1 and β 2). The TRα1 and TRα2 isoforms are the result of alternative splicing from the primary transcript of the c-erbA-α gene. The expression of these receptors changes as a result of various devel
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Detection of Three Major Polymorphisms in the ,-Acetyltransferase 2 Gene by Melting Peak Analysis Usd that the marked interindividual variation in the elimination of isoniazid was due to the genetically controlled expression of the .-Acetyltransferase 2 enzyme (NAT2). The so-called slow acetylators of isoniazid elimination were homozygous for a recessive gene, whereas rapid acetylators were either
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Fluorescent Hybridization Probe Detection of the F508del Cystic Fibrosis Allele on the LightCycler The cystic fibrosis transconductance regulator gene that is altered in the disease is on chromosome 7 [2]. Population screening has uncovered nearly 900 variants in the gene to date [3]. Many of these are disease-causing mutations. Approximately 70% of disease-causing alleles have a 3 base pair del
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Simultaneous Detection of C282Y and H63D Hemochromatosis Mutations Using LCRed 640 and LCRed 705 Lab with a frequency of approximately 0.5% in Caucasian populations (1). Hemochromatosis leads to organ failure due to iron accumulation and can be misdiagnosed as heart or liver disease. Serious complications include cirrhosis, hepatomas, diabetes, cardiomyopathy, arthritis and hypogonadotrophic hypog
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