Titlebook: Rab GTPases; Methods and Protocol Guangpu Li Book 2015 Springer Science+Business Media New York 2015 Endocytic Pathways.Eukaryotes.Exocytic

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Measuring Rab GTPase-Activating Protein (GAP) Activity in Live Cells and Extracts,ing particular Rabs as substrates for their cognate GTPase-activating proteins. However, in vitro activity does not always reflect substrate specificity in cells. This method describes a functional test of GAP activity in cells or extracts that takes into account the presence of other factors or con

古董

Analysis of the Interactions Between Rab GTPases and Class V Myosins,ell division. Humans have over 40 myosins that can be placed into 18 classes, the malfunctioning of a number of which can lead to disease. There are three members of the human class V myosin family, myosins Va, Vb, and Vc. People lacking functional myosin Va suffer from a rare autosomal recessive di

Postulate

Intervention

Kinetic Activation of Rab8 Guanine Nucleotide Exchange Factor Rabin8 by Rab11,from the .-Golgi network (TGN) and recycling endosomes to the plasma membrane. Rabin8 is a major guanine nucleotide exchange factor (GEF) for Rab8. It activates Rab8 by catalyzing its GDP release for subsequent GTP loading. However, how Rabin8 itself is activated in cells is unclear. Recently, it wa

CURB

Ypt1 and TRAPP Interactions: Optimization of Multicolor Bimolecular Fluorescence Complementation inare stimulated by guanine-nucleotide exchange factors (GEFs). In yeast, Ypt1 regulates transport from the endoplasmic reticulum (ER) to two alternative pathways: secretion and autophagy. Ypt1 is activated by TRAPP, a modular multi-subunit GEF. Whereas TRAPP I activates Ypt1 to mediate transport thro

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Identifying a Rab Effector on the Macroautophagy Pathway,, and the macroautophagy pathway. To identify effectors on the macroautophagy pathway, known autophagy-related genes (Atg genes) required for macroautophagy were tagged with GFP and screened for mislocalization in the .-. mutant. At the pre-autophagosomal structure (PAS), the localization of the ser

CRUMB

Albinism

Small GTPases in Acrosomal Exocytosis,ptor (SNARE) and Rab superfamilies are members of this machinery. Rab proteins are small GTPases that organize membrane microdomains on organelles by recruiting specific effectors that strongly influence the movement, fusion and fission dynamics of intracellular compartments. Rab3 and Rab27 are the

CORE

Rab Antibody Characterization: Comparison of Rab14 Antibodies,ll junctions to the plasma membrane. It localizes predominantly to endocytic membranes with a pool also found on .-Golgi network (TGN) membranes, and is most closely related to the Rab11 subfamily of GTPases. Certain intracellular bacteria such as ., ., and . utilize Rab14 to promote their maturatio

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Selective Visualization of GLUT4 Storage Vesicles and Associated Rab Proteins Using IRAP-pHluorin,e the cell. However, it was difficult to distinguish GLUT4 storage vesicles (GSVs) from other intracellular compartments containing GLUT4 in live cells. Here, we describe the use of IRAP-pHluorin and total internal reflection fluorescence (TIRF) microscopy to selectively visualize GSVs and Rab prote