Titlebook: RT-PCR Protocols; Joe O’Connell Book 20021st edition Humana Press 2002

carotenoids

Quantitation of Gene Expression by RT-PCR and HPLC Analysis of PCR Productstive method requiring microgram amounts of RNA. It is time consuming and semi-quantitative at best. Because of the limitations of Northern blotting, various strategies have been developed for quantitation of cDNA by polymerase chain reaction (PCR)-based methods (.–.). Competitive PCR, in which a syn

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BLOT

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一起平行

Semi-Quantitative Detection of Hepatitis C Virus RNA by “Real-Time” RT-PCRol to tailor and monitor antiviral therapies for this disease (.). Although a low viral load is associated with a higher efficacy of Interferonm (IFN)-α/ribavirin combination therapies, patients with a high viral load respond more poorly to this treatment and require a longer duration of therapy. In

Ablation

RT-PCR for the Assessment of Genetically Heterogenous Populations of the Hepatitis C Virusen an intensive focus on understanding the underlying biology of its disease process. Of significant interest has been the study of heterogenous populations of closely related, but genetically non-identical HCV virions, commonly termed quasispecies (.,.), and their relationship to the pathogenesis o

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STERN

Quantitative RT-PCRverse transcription, and amplification of a specific cDNA by polymerase chain reaction (PCR). The method requires very little RNA and differs from Northern blotting because it is somewhat tolerant of degraded RNA, as long as the RNA is intact within the region of interest.

变白

Detection and Quantification of the Hepatitis C Viral Genomeen instrumental in assessing the natural history of HCV, in which viral RNA levels have a large dynamic range. Assessment of the HCV viral load is now a routine part of the algorithm for the diagnosis of chronic hepatitis C (.–.). Patient’s response to anti-viral therapy can be assessed, in part, by quantification of serum viral loads (.,.).