| 书目名称 | RT-PCR Protocols | | 副标题 | Second Edition | | 编辑 | Nicola King | | 视频video | | | 概述 | Features all the latest and hottest technology used in conventional and real-time RT-PCR.Provides a broad scope covered in good depth, from detection of minute qualities of microRNA to the analysis of | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | .Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In .RT-PCR Protocols, Second Edition., expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful .Methods in Molecular Biology.™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls...User friendly and up-to-date, .RT-PCR Protocols, Second Edition. acts as a handy companion to scientists from numerous diverse backgrounds who wish to | | 出版日期 | Book 2010Latest edition | | 关键词 | Cloning; Gene sequences; PCR; Primer design; Real-time quantitative RT-PCR; Reverse transcription; Reverse | | 版次 | 2 | | doi | https://doi.org/10.1007/978-1-60761-629-0 | | isbn_softcover | 978-1-4939-5682-1 | | isbn_ebook | 978-1-60761-629-0Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC 2010 |
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发表于 2025-3-21 19:43:14
