| 书目名称 | RNAi and Small Regulatory RNAs in Stem Cells | | 副标题 | Methods and Protocol | | 编辑 | Baohong Zhang | | 视频video | | | 概述 | Includes cutting-edge methods and protocols for the study of the intersection of RNAi, small regulatory RNAs, and stem cell biology.Provides step-by-step detail essential for reproducible results.Cont | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | This detailed collection provides an accessible compendium of up-to-date methods focused on the study of RNAi and small regulatory miRNAs in stem cells. Beginning with a brief introductory section, the volume continues by exploring methods and protocols for RNAi screening, transfection, and the knockdown of specific genes and pathways in several animal species, including humans and mice, recently developed methods for miRNA expression and functional analysis, as well as usage of CRISPR/Cas 9 to knockout an individual gene for functional studies. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Practical and authoritative, .RNAi and Small Regulatory RNAs in Stem Cells: Methods and Protocols. aims to accelerate progress in this crucial field by reducing the time required to decipher and put into practice procedures published in the literature.. | | 出版日期 | Book 2017 | | 关键词 | Stem cell differentiation; MicroRNAs; RNA interference; CRISPR-Cas9; Regenerative medicine; Gene knockdow | | 版次 | 1 | | doi | https://doi.org/10.1007/978-1-4939-7108-4 | | isbn_softcover | 978-1-4939-8386-5 | | isbn_ebook | 978-1-4939-7108-4Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Springer Science+Business Media, LLC, part of Springer Nature 2017 |
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发表于 2025-3-21 17:30:56
