Titlebook: RNAi Strategies for Pest Management; Methods and Protocol Luis María Vaschetto Book 2022 The Editor(s) (if applicable) and The Author(s), u

使厌恶

In Silico Screening of Attractive Target Genes for RNAi-Based Pest Control, genes is a crucial initial step. In this chapter, I describe the crucial procedure of in silico screening of the most a5ttractive target genes for RNAi-based pest control, including gene or protein homology comparison, specificity optimization of the dsRNAs to target pests to minimize off-target effect, and primer design.

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Visualization of RNA Transcripts in Western Corn Rootworm (, and Plants by In Situ Hybridization,ctioning, target recovery, and chromogenic detection of an mRNA (DvSSJ1), encoding for a membrane protein associated with the smooth septate junction (SSJ) in Western corn rootworm [.]. Further, we demonstrate, the expression of dsRNA of DvSSJ1 in maize root tissues using signal amplification and background suppression technology.

保守

Enrage

巨硕

Functional Characterization of Silkworm PIWI Proteins by Embryonic RNAi,A-injected single embryo. The siRNA-injected embryo can be molecularly sexed by W chromosome-specific DNA markers. Using complementary DNA (cDNA) reverse transcribed from the sexed RNA, we evaluated the knockdown effect of the PIWI protein-coding genes on a sexual development-related gene, ..

清晰

步兵

mucous-membrane

Functional Characterization of Target Genes Associated with Insecticide Resistance of the Green Peaptibility of RNAi-treated populations compared to controls, the target gene may have a direct role in the development of resistance to the insecticide or the gene may be involved in other metabolic processes that may be required for resilience against the insecticide.

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