Titlebook: RNA Vaccines; Methods and Protocol Thomas Kramps,Knut Elbers Book 2017 Springer Science+Business Media New York 2017 recombinant viruses.se

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Electroporation of mRNA as Universal Technology Platform to Transfect a Variety of Primary Cells wittypes. We have spent more than a decade to optimize and adapt this method, first for antigen-loading of dendritic cells (DCs), and subsequently for T cells, B cells, bulk PBMCs, and several cell lines. In this regard, antigens were introduced, processed, and presented in context of MHC class I and I

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Adjuvant-Enhanced mRNA Vaccinesent in its translational efficacy. Nowadays, mRNA-based vaccines represent a promising approach in the field of anticancer immunotherapy, gaining attention over the earlier-established bacteria-, virus-, or cell-based vaccination approaches. Here, we present the experimental procedures employed in o

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