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Titlebook: RNA Vaccines; Methods and Protocol Thomas Kramps Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive l

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发表于 2025-3-21 17:11:57 | 显示全部楼层 |阅读模式
书目名称RNA Vaccines
副标题Methods and Protocol
编辑Thomas Kramps
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Vaccines; Methods and Protocol Thomas Kramps Book 2024Latest edition The Editor(s) (if applicable) and The Author(s), under exclusive l
描述This second edition volume expands on the previous edition with discussions of the latest technology and advancements in the development of RNA vaccines and proposed solutions on how to tackle currently unmet medical needs. The chapters in this book are organized into four parts and cover topics such as replication and non-replicating RNA vectors, formulation and delivery of RNA vaccines, and clinical and nonclinical development of RNA vaccines. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Practical and comprehensive, .RNA Vaccines: Methods and Protocols, Second Edition. is a valuable resource for any researcher interested in learning more about this important and developing field.  .
出版日期Book 2024Latest edition
关键词RNA vectors; Vaccine delivery; Preclinical development; Clinical development; Adjuvant activity
版次2
doihttps://doi.org/10.1007/978-1-0716-3770-8
isbn_softcover978-1-0716-3772-2
isbn_ebook978-1-0716-3770-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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发表于 2025-3-22 00:18:00 | 显示全部楼层
Negative-Strand RNA Virus-Vectored Vaccinesr . with special attention to Newcastle disease virus-based vaccines and their generation. It shall provide an overview on the advantages and disadvantages of certain vector platforms as well as their scopes of application, including an additional section on experimental COVID-19 vaccines.
发表于 2025-3-22 01:19:20 | 显示全部楼层
Regulatory Considerations for Producing mRNA Vaccines for Clinical Trialsnew drug (IND) application. For that, several preconditions concerning production, quality control, and assurance have to be fulfilled. Here, specific requirements related to mRNA vaccines are addressed on the basis of European standards.
发表于 2025-3-22 07:42:12 | 显示全部楼层
Plant Expression of ,-Encapsidated Chimeric Viral Vaccines with Animal RNA Replicons: An Updatepolyethylene glycol purification protocol is also provided to collect and purify these vaccines for immunological tests. In this update, we also provide a protocol for . co-inoculation of a modified FHV protein A, which significantly increased the yield of . chimeric viral vaccine.
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Generation and Characterization of In Vitro Transcribed mRNAlecules. For the in vitro testing of the mRNA molecules, we include protocols for the transfection of various primary cell cultures and the confirmation of translation by intracellular staining and western blotting.
发表于 2025-3-22 18:27:14 | 显示全部楼层
Sequence-Optimized mRNA Vaccines Against Infectious Diseasearticles. Methods described here include the synthesis, purification, and formulation of mRNA vaccines as well as a comprehensive panel of in vitro and in vivo methods for evaluation of vaccine quality and immunogenicity.
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Introduction to RNA Vaccines Post COVID-19,re effectively countering newly emerging pathogens, fighting currently intractable infections, or even generating novel treatment modalities for non-infectious diseases. Instead of applying protein antigen directly, RNA vaccines contain short-lived genetic information that guides the expression of p
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