Titlebook: RNA Structure and Dynamics; Jienyu Ding,Jason R. Stagno,Yun-Xing Wang Book 2023 This is a U.S. government work and not under copyright pro

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Methods in Molecular Biologyhttp://image.papertrans.cn/r/image/820197.jpg

察觉

https://doi.org/10.1007/978-1-0716-2687-0X-ray Crystallography; XFEL; riboswitches; NMR; RNA conformational trajectory

表皮

Characterizing Fluorescence Properties of Turn-on RNA Aptamers,s should be characterized prior to cellular imaging experiments. Here, we describe the process of determining the fluorophore affinity, fluorescence enhancement, and fluorescence lifetime(s) of the Mango-III fluorescence turn-on aptamer. Parameters determined through these protocols will aid in establishing conditions for live-cell imaging.

成绩上升

Isothermal Titration Calorimetry Analysis of a Cooperative Riboswitch Using an Interdependent-Sites alteration of the underlying chemical structures. In this protocol, we describe procedures for performing, analyzing, and interpreting ITC data obtained from a cooperative riboswitch–ligand interaction.

Meager

Hamper

Probing Transient Riboswitch Structures via Single Molecule Accessibility Analysis, monitored by Total Internal Reflection Fluorescence Microscopy (TIRFM) and quantitatively analyzed, via spike train and burst detection, to elucidate the rate constants of isomerization, revealing mechanistic insights into riboswitching.

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Narrative

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