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Titlebook: RNA Structure Determination; Methods and Protocol Douglas H. Turner,David H. Mathews Book 2016 Springer Science+Business Media New York 201

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发表于 2025-3-21 18:48:44 | 显示全部楼层 |阅读模式
书目名称RNA Structure Determination
副标题Methods and Protocol
编辑Douglas H. Turner,David H. Mathews
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts.Includes supplementary materia
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Structure Determination; Methods and Protocol Douglas H. Turner,David H. Mathews Book 2016 Springer Science+Business Media New York 201
描述.This volume provides protocols and procedures for determining and modeling RNA structure.  Chapters guide the reader through protocols for RNA secondary structure prediction, single sequence modeling, Crumple,  RNAstructure to model conserved secondary structures with multiple homologs,  the prediction of bimolecular secondary structures with RNAstructure, STarMir, protocols for structure mapping, mapping data to constrain or restrain RNA secondary structure prediction with RNAstructure, unassigned NMR resonances, modeling protocols for Rosetta FARFAR, RNAComposer , ModeRNA, and MC-Fold. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls... ..Authoritative and Practical, .RNA Structure Determination:Methods and Protocols. aims to ensure successful results in the further study of this vital field..
出版日期Book 2016
关键词RNA secondary structure prediction; single sequence modeling; RNAstructure; structure mapping; NMR reson
版次1
doihttps://doi.org/10.1007/978-1-4939-6433-8
isbn_softcover978-1-4939-8198-4
isbn_ebook978-1-4939-6433-8Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2016
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发表于 2025-3-21 21:09:07 | 显示全部楼层
Secondary Structure Prediction of Single Sequences Using RNAstructure, energy minimization, prediction of base pairing probabilities, prediction of structures composed of highly probably base pairs, and prediction of structures with pseudoknots. A user-friendly graphical user interface is provided, and this interface works on Windows, Apple OS X, and Linux. This chapt
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Prediction of Secondary Structures Conserved in Multiple RNA Sequences, much more accurate than predicting the structure for a single sequence. RNAstructure is a software package that includes the programs Dynalign, Multilign, TurboFold, and PARTS for predicting conserved RNA secondary structure. This chapter provides protocols for using these programs.
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,Predicting RNA–RNA Interactions Using RNAstructure,ause of the competition between intramolecular and intermolecular structure formation. A complete picture of RNA–RNA binding includes an understanding of single-stranded folding and binding site accessibility, and is strongly concentration-dependent. This chapter provides guidance for using RNAstruc
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Traditional Chemical Mapping of RNA Structure In Vitro and In Vivo,gands. The method monitors the reactivity of each nucleotide towards chemicals of various specificities reflecting the hydrogen bonding environment of each nucleotide within the RNA molecule. In addition, information can be obtained on the binding site of a ligand (noncoding RNAs, protein, metabolit
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High-Throughput Nuclease Probing of RNA Structures Using FragSeq,ultaneously. Such assays accelerate RNA structure analysis and provide researchers with new technologies to tackle biological questions on a transcriptome-wide scale. FragSeq is an experimental assay for transcriptome-wide RNA structure probing using RNA-Seq, coupled with data analysis tools that al
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Experiment-Assisted Secondary Structure Prediction with RNAstructure,age of enzymatic cleavage data, FMN cleavage data, traditional chemical modification reactivity data, and SHAPE reactivity data for secondary structure modeling. This chapter provides protocols for using experimental probing data with RNAstructure to restrain or constrain RNA secondary structure pre
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