Titlebook: RNA Isolation and Characterization Protocols; Ralph Rapley,David L. Manning Book 1998 Springer Science+Business Media New York 1998

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Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides,eing a difficult source from which to isolate high-quality RNA with good yield. This difficulty is primarily due to the presence of naturally occurrmg polysaccharides and/or polyphenols that are released during cell disruption. These compounds form complexes with nucleic acids during tissue extracti

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Isolation of Plant Mitochondrial RNA from Green Leaves,fied mtRNA is necessary not only for construction of a mitochondrial cDNA library, but also for the analysis of plant mitochondrial transcription. Several methods have been frequently used for isolation of plant mtRNA (.–.). However, these mtRNA preparations may be heavily contaminated by chloroplas

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Extraction of RNA from Fresh and Frozen Blood,ogemzation as is necessary with solid tissues. However, blood is a particularly problematic tissue from which to isolate RNA because RNA is extremely prone to degradation by ribonucleases, of which red cells are a rich source. Furthermore, blood constituents or their derivatives may inhibit PCR reac

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Isolation of Total RNA from Bacteria,sually occurs complexed with protein from which it must be released. Precautions to be taken against exogenous RNase include the use of plastic gloves, autoclavmg solutions after adding 0.1% (v/v) diethyl pyrocarbonate (DEPC; except Trrs, which reacts), and baking glassware, spatulas, and so forth a

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UV Spectrophotometric Analysis of Ribonucleic Acids,the majority of situations this is carried out using spectrophotometry, which is nondestructive and allows the sample to be recovered for further analysis or manipulation. Spectrophotometry makes use of the fact that there is a relationship between the absorption of ultraviolet light by RNA and its

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Interlocking

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Nonradioactive Northern Blotting,ein,” and “biotin” which are linked through a spacer to a nucleotide (in most cases UTP or dUTP) and are incorporated into specific gene-probes by various methods (e.g., random-priming, nick-translation, or PCR). The detection is based on the specific interaction of the labels with appropriate prote

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