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Titlebook: RNA Abundance Analysis; Methods and Protocol Hailing Jin,Isgouhi Kaloshian Book 2021Latest edition Springer Science+Business Media, LLC, pa

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书目名称RNA Abundance Analysis
副标题Methods and Protocol
编辑Hailing Jin,Isgouhi Kaloshian
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: RNA Abundance Analysis; Methods and Protocol Hailing Jin,Isgouhi Kaloshian Book 2021Latest edition Springer Science+Business Media, LLC, pa
描述This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .RNA Abundance Analysis: Methods and Protocols, Second Edition. provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies..
出版日期Book 2021Latest edition
关键词mRNAs; Small RNAs; Gene expression; RNA expression; Next-generation sequencing; RNA-protein interactions
版次2
doihttps://doi.org/10.1007/978-1-0716-0743-5
isbn_softcover978-1-0716-0745-9
isbn_ebook978-1-0716-0743-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2021
The information of publication is updating

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CRISPR-Cas RNA Targeting Using Transient Cas13a Expression in ,g. The class 2 Type VI CRISPR-Cas13 system is an RNA-guided RNA-nuclease system capable of binding and cleaving target single-stranded RNA substrates in a sequence-specific manner. In addition to RNA interference, the Cas13a system has application from manipulating RNA modifications, to editing RNA
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Laser Microdissection of Cells and Isolation of High-Quality RNA After Cryosectioning,s. Widely used in animal research, still few studies on plants have been carried out. We have applied this technique to the plant–nematode interaction by isolating feeding cells (giant cells; GCs) immersed inside complex swelled root structures (galls) induced by root-knot nematodes. For this purpos
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Quantitative Analysis of Plant miRNA Primary Transcripts, controlled since both excess and deficiency of a given microRNA may be deleterious to plant cell. MicroRNA expression regulation can occur at several stages of their biogenesis pathway. One of the most important of these regulatory checkpoints is transcription efficiency. mirEX database is a tool f
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,Analysis of RBP Regulation and Co-regulation of mRNA 3′ UTR Regions in a Luciferase Reporter Systemelements can be fused to the luciferase gene, and as a result effect protein output by changing rates of transcription, rates of translation, or mRNA stability. This protocol focuses on probing the function of RNA-binding proteins (RBPs) through their interactions with the 3′ untranslated region (UT
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